Prof. Dr. Francesca Paradisi

Journal Article

Broumidis, Emmanouil; Paradisi, Francesca (2024). Engineering a Dual-Functionalized PolyHIPE Resin for Photobiocatalytic Flow Chemistry. Angewandte Chemie. International edition, 63(21), e202401912. Wiley 10.1002/anie.202401912

Gil-Garcia, Marcos; Benítez-Mateos, Ana I; Papp, Marcell; Stoffel, Florence; Morelli, Chiara; Normak, Karl; Makasewicz, Katarzyna; Faltova, Lenka; Paradisi, Francesca; Arosio, Paolo (2024). Local environment in biomolecular condensates modulates enzymatic activity across length scales. Nature communications, 15(3322) Nature Publishing Group 10.1038/s41467-024-47435-w

Díaz-Kruik, Pablo; Paradisi, Francesca (2024). Rapid production of the anaesthetic mepivacaine through continuous, portable technology. Green chemistry, 26(4), pp. 2313-2321. Royal Society of Chemistry 10.1039/d3gc04375d

Benítez-Mateos, Ana I.; Paradisi, Francesca (2024). Perspectives on flow biocatalysis: the engine propelling enzymatic reactions. Journal of flow chemistry, 14(1), pp. 211-218. Springer 10.1007/s41981-023-00283-z

Roura Padrosa, David; Wetzl, Dennis; Hildbrand, Stefan; Tosatti, Paolo; Sedelmeier, Joerg; Puentener, Kurt; Iding, Hans; Paradisi, Francesca (2024). Biocatalyst Immobilization and Process Intensification: Production of ( S )-Piperazine-2-carboxylic Acid Dihydrochloride. Organic process research & development, 28(5), pp. 1713-1724. ACS Publications 10.1021/acs.oprd.3c00347

Stalder, Kaja; Benitez-Mateos, Ana I.; Paradisi, Francesca (2024). Biocatalytic Syntheis of L‐Pipecolic Acid by a Lysine Cyclodeaminase: Batch and Flow Reactors. ChemCatChem, 16(12) WILEY-VCH 10.1002/cctc.202301671

Contaldo, Umberto; Roura Padrosa, David; Jamet, Hélène; Albrecht, Martin; Paradisi, Francesca; Le Goff, Alan (2023). Optimising Electrical Interfacing between the Trimeric Copper Nitrite Reductase and Carbon Nanotubes. Chemistry: a European journal, 29(47), e202301351. Wiley 10.1002/chem.202301351

Lim, David; Paradisi, Francesca (2023). Investigations into the Aqueous Synthesis of Selenoglycoconjugates. European journal of organic chemistry, 26(34) Wiley-VCH 10.1002/ejoc.202300496

Roura Padrosa, David; Paradisi, Francesca (2023). Bioinformatic analysis of immobilized enzymes: towards a better understanding of the guiding forces. ChemBioChem, 24(11), e202200723. Wiley 10.1002/cbic.202200723

Benítez-Mateos, Ana I; Paradisi, Francesca (2023). Halomonas elongata: a microbial source of highly stable enzymes for applied biotechnology. Applied microbiology and biotechnology, 107(10), pp. 3183-3190. Springer 10.1007/s00253-023-12510-7

Paradisi, Francesca (2023). Producing alanine from methanol with a new cell-free synthetic pathway. Chem Catalysis, 3(3), p. 100533. Elsevier 10.1016/j.checat.2023.100533

Meersseman Arango, Hippolyte; van den Biggelaar, Ludivine; Soumillion, Patrice; Luis, Patricia; Leyssens, Tom; Paradisi, Francesca; Debecker, Damien P. (2023). Continuous flow-mode synthesis of (chiral) amines with transaminase: a strategic biocatalytic approach to essential building blocks. Reaction chemistry & engineering, 8(7), pp. 1505-1544. Royal Society of Chemistry 10.1039/D3RE00210A

Roura Padrosa, David; Lehmann, Hansjoerg; Snajdrova, Radka; Paradisi, Francesca (2023). Sustainable synthesis of L-phenylalanine derivatives in continuous flow by immobilized phenylalanine ammonia lyase. Frontiers in Catalysis, 3 Frontiers Media 10.3389/fctls.2023.1147205

Díaz-Kruik, Pablo; Gianolio, Stefania; Paradisi, Francesca (2023). Flow chemistry Set-up Enables Integration of Chemo- and Biocatalysis. CHIMIA, 77(5), pp. 307-311. Schweizerische Chemische Gesellschaft 10.2533/chimia.2023.307

Robustini, Lucia; Paradisi, Francesca (2023). Enanatioselective Switch and Potential Applications in Biocatalysis. CHIMIA, 77(6), pp. 390-394. Schweizerische Chemische Gesellschaft 10.2533/chimia.2023.390

De Lorenzo, Sabrina; Pillet, Lauriane; Lim, David; Paradisi, Francesca (2023). Glycosyl Benzoates as Novel Substrates for Glycosynthases. Organic & biomolecular chemistry, 21(31), pp. 6356-6359. Royal Society of Chemistry 10.1039/D3OB00979C

Donzella, Silvia; Compagno, Concetta; Molinari, Francesco; Paradisi, Francesca; Contente, Martina Letizia (2023). Boosting the catalytic performance of a marine yeast in a SpinChem ® reactor for the synthesis of perillyl alcohol. Reaction chemistry & engineering, 8(12), pp. 2963-2966. Royal Society of Chemistry 10.1039/D3RE00474K

Mero, Angelica; Moody, Nicholas R.; Husanu, Elena; Mezzetta, Andrea; D’Andrea, Felicia; Pomelli, Christian Silvio; Bernaert, Nathalie; Paradisi, Francesca; Guazzelli, Lorenzo (2023). Challenging DESs and ILs in the valorization of food waste: a case study. Frontiers in Chemistry, 11 Frontiers Media 10.3389/fchem.2023.1270221

Marchini, Valentina; Benitez Mateos, Ana Isabel; Hutter, Sofia; Paradisi, Francesca (2022). Fusion of Formate Dehydrogenase and Alanine Dehydrogenase as an Amino Donor Regenerating System Coupled to Transaminases. ChemBioChem, 23(21), e202200428. Wiley-VCH 10.1002/cbic.202200428

Benítez-Mateos, Ana I; Schneider, Andreas; Hegarty, Eimear; Hauer, Bernhard; Paradisi, Francesca (2022). Spheroplasts preparation boosts the catalytic potential of a squalene-hopene cyclase. Nature Communications, 13(1), p. 6269. Springer Nature 10.1038/s41467-022-34030-0

Romero-Fernandez, Maria; Heckmann, Christian M; Paradisi, Francesca (2022). Biocatalytic production of a Nylon 6 precursor from caprolactone in continuous flow. ChemSusChem, 15(16), e202200811. Wiley 10.1002/cssc.202200811

Benitez-Mateos, Ana I; Klein, Calvin; Roura Padrosa, David; Paradisi, Francesca (2022). A novel thymidine phosphorylase to synthesize (halogenated) anticancer and antiviral nucleoside drugs in continuous flow. Catalysis science & technology, 12(20), pp. 6231-6238. Royal Society of Chemistry 10.1039/D2CY00751G

Heckmann, Christian M; Robustini, Lucia; Paradisi, Francesca (2022). Influence of reaction conditions on enzymatic enantiopreference: the curious case of HEwT in the synthesis of THF-amine. ChemBioChem, 23(15), e202200335. Wiley 10.1002/cbic.202200335

Benítez-Mateos, Ana I; Roura Padrosa, David; Paradisi, Francesca (2022). Multistep enzyme cascades as a route towards green and sustainable pharmaceutical syntheses. Nature chemistry, 14(5), pp. 489-499. Springer Nature 10.1038/s41557-022-00931-2

Romero-Fernandez, Maria; Paradisi, Francesca (2022). Stereo‐divergent enzyme cascades to convert racemic 4‐phenyl‐2‐butanol into either (S)‐ or (R)‐ corresponding chiral amine. ChemBioChem, 23(8), e202200108. Wiley-VCH 10.1002/cbic.202200108

Byrne, Joseph P; Delgado, Lidia; Paradisi, Francesca; Albrecht, Martin (2022). Carbohydrate‐Functionalized Triazolylidene Iridium Complexes: Hydrogenation Catalysis in Water with Asymmetric Induction. ChemCatChem, 14(7), e202200086. WILEY-VCH 10.1002/cctc.202200086

Contente, Martina L.; Marzuoli, Irene; Iding, Hans; Wetzl, Dennis; Puentener, Kurt; Hanlon, Steven P.; Paradisi, Francesca (2022). Screening methods for enzyme-mediated alcohol oxidation. Scientific reports, 12(1), p. 3019. Springer Nature 10.1038/s41598-022-07008-7

Alvarez, Elena; Romero-Fernandez, Maria; Iglesias, Diego; Martinez-Cuenca, Raul; Okafor, Obinna; Delorme, Astrid; Lozano, Pedro; Goodridge, Ruth; Paradisi, Francesca; Walsh, Darren A.; Sans, Victor (2022). Electrochemical Oscillatory Baffled Reactors Fabricated with Additive Manufacturing for Efficient Continuous-Flow Oxidations. ACS sustainable chemistry & engineering, 10(7), pp. 2388-2396. American Chemical Society 10.1021/acssuschemeng.1c06799

Benitez Mateos, Ana I.; Paradisi, Francesca (2022). Sustainable flow‐synthesis of (bulky)nucleoside drugs by a novel and highly stable nucleoside phosphorylase immobilized on reusable supports. ChemSusChem, 15(1), e202102030. Wiley-VCH 10.1002/cssc.202102030

Pillet, Lauriane; Lim, David; Almulhim, Nourah; Benítez-Mateos, Ana I; Paradisi, Francesca (2022). Novel triple mutant of an extremophilic glycosyl hydrolase enables the rapid synthesis of thioglycosides. Chemical communications, 58(86), pp. 12118-12121. Royal Society of Chemistry 10.1039/D2CC04660A

Gianolio, Stefania; Roura Padrosa, David; Paradisi, Francesca (2022). Combined chemoenzymatic strategy for sustainable continuous synthesis of the natural product hordenine. Green chemistry, 24(21), pp. 8434-8440. Royal Society of Chemistry 10.1039/D2GC02767D

Heckmann, Christian M.; Paradisi, Francesca (2021). GPhos Ligand Enables Production of Chiral N ‐Arylamines in a Telescoped Transaminase‐Buchwald‐Hartwig Amination Cascade in the Presence of Excess Amine Donor. Chemistry - a European journal, 27(67), pp. 16616-16620. Wiley-VCH 10.1002/chem.202103472

Marchini, Valentina; Benítez Mateos, Ana I.; Roura Padrosa, David; Paradisi, Francesca (2021). Fusion of Glutamate Dehydrogenase and Formate Dehydrogenase Yields a Bifunctional Efficient Biocatalyst for the Continuous Removal of Ammonia. Frontiers in Catalysis, 1 Frontiers Media 10.3389/fctls.2021.790461

Roura Padrosa, David; Marchini, Valentina; Paradisi, Francesca (2021). CapiPy: python based GUI-application to assist in protein immobilization. Bioinformatics, 37(17), pp. 2761-2762. Oxford University Press 10.1093/bioinformatics/btab030

Montanari, Ulisse; Cocchi, Davide; Brugo, Tommaso Maria; Pollicino, Antonino; Taresco, Vincenzo; Romero Fernandez, Maria; Moore, Jonathan C.; Sagnelli, Domenico; Paradisi, Francesca; Zucchelli, Andrea; Howdle, Steven M.; Gualandi, Chiara (2021). Functionalisable Epoxy-rich Electrospun Fibres Based on Renewable Terpene for Multi-Purpose Applications. Polymers, 13(11), p. 1804. MDPI 10.3390/polym13111804

Roura Padrosa, David; Nisar, Zoya; Paradisi, Francesca (2021). Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors. Catalysts, 11(4), p. 520. MDPI 10.3390/catal11040520

Heckmann, Christian M.; Dominguez, Beatriz; Paradisi, Francesca (2021). Enantio-Complementary Continuous-Flow Synthesis of 2-Aminobutane Using Covalently Immobilized Transaminases. ACS sustainable chemistry & engineering, 9(11), pp. 4122-4129. American Chemical Society 10.1021/acssuschemeng.0c09075

Delgado, Lidia; Heckmann, Christian M.; Di Pisa, Flavio; Gourlay, Louise; Paradisi, Francesca (2021). Release of Soybean Isoflavones by Using a β‐Glucosidase from Alicyclobacillus herbarius. ChemBioChem, 22(7), pp. 1223-1231. Wiley-VCH 10.1002/cbic.202000688

Delgado, Lidia; Heckmann, Christian M.; De Benedetti, Stefano; Nardini, Marco; Gourlay, Louise J.; Paradisi, Francesca (2021). Producing natural vanilla extract from green vanilla beans using a β-glucosidase from Alicyclobacillus acidiphilus. Journal of biotechnology, 329, pp. 21-28. Elsevier 10.1016/j.jbiotec.2021.01.017

Benítez-Mateos, Ana I.; Contente, Martina L.; Roura Padrosa, David; Paradisi, Francesca (2021). Flow biocatalysis 101: design, development and applications. Reaction chemistry & engineering, 6(4), pp. 599-611. Royal Society of Chemistry 10.1039/D0RE00483A

Romero-Fernandez, Maria; Paradisi, Francesca (2021). Biocatalytic access to betazole by one-pot multienzymatic system in continuous flow. Green chemistry, 23(12), pp. 4594-4603. Royal Society of Chemistry 10.1039/D1GC01095F

Benítez-Mateos, Ana I.; Bertella, Stefania; Behaghel de Bueren, Jean; Luterbacher, Jeremy S.; Paradisi, Francesca (2021). Dual Valorization of Lignin as a Versatile and Renewable Matrix for Enzyme Immobilization and (Flow) Bioprocess Engineering. ChemSusChem, 14(15), pp. 3198-3207. Wiley-VCH 10.1002/cssc.202100926

Lenzen, Karst; Planchestainer, Matteo; Feller, Isabelle; Roura Padrosa, David; Paradisi, Francesca; Albrecht, Martin (2021). Minimalistic peptidic scaffolds harbouring an artificial carbene-containing amino acid modulate reductase activity. Chemical communications, 57(72), pp. 9068-9071. Royal Society of Chemistry 10.1039/D1CC03158A

Planchestainer, Matteo; McMaster, Jonathan; Schulz, Christine; Paradisi, Francesca; Albrecht, Martin (2020). Carbene‐Induced Rescue of Catalytic Activity in Deactivated Nitrite Reductase Mutant. Chemistry - a European journal, 26(66), pp. 15206-15211. Wiley-VCH 10.1002/chem.202002444

Hegarty, Eimear; Paradisi, Francesca (2020). Implementation of Biocatalysis in Continuous Flow for the Synthesis of Small Cyclic Amines. CHIMIA, 74(11), pp. 890-894. Schweizerische Chemische Gesellschaft 10.2533/chimia.2020.890

Contente, Martina L.; Fiore, Noemi; Cannazza, Pietro; Roura Padrosa, David; Molinari, Francesco; Gourlay, Louise; Paradisi, Francesca (2020). Uncommon overoxidative catalytic activity in a new halo‐tolerant alcohol dehydrogenase. ChemCatChem, 12(22), pp. 5679-5685. WILEY-VCH 10.1002/cctc.202001112

Heckmann, Christian M.; Gourlay, Louise J.; Dominguez, Beatriz; Paradisi, Francesca (2020). An (R)-Selective Transaminase From Thermomyces stellatus: Stabilizing the Tetrameric Form. Frontiers in Bioengineering and Biotechnology, 8(707) Frontiers Media 10.3389/fbioe.2020.00707

Roura Padrosa, David; Benítez-Mateos, Ana I.; Calvey, Liam; Paradisi, Francesca (2020). Cell-free biocatalytic syntheses of L-pipecolic acid: a dual strategy approach and process intensification in flow. Green chemistry, 22(16), pp. 5310-5316. Royal Society of Chemistry 10.1039/D0GC01817A

ul Haque, R; Allers, T; Paradisi, Francesca (2020). Haloferax volcanii for biotechnology applications: challenges, current state and perspectives. Applied microbiology and biotechnology, 104(4), pp. 1371-1382. Springer 10.1007/s00253-019-10314-2

Contente, Martina Letizia; Tamborini, Lucia; Molinari, Francesco; Paradisi, Francesca (2020). Aromas flow: eco-friendly, continuous, and scalable preparation of flavour esters. Journal of flow chemistry, 10(1), pp. 235-240. Springer 10.1007/s41981-019-00063-8

Almulhim, Nouarh; Moody, Nicholas R.; Paradisi, Francesca (2020). Engineering novel S-glycosidase activity into extremo-adapted β-glucosidase by rational design. Applied microbiology and biotechnology, 104(10), pp. 4407-4415. Springer 10.1007/s00253-020-10582-3

Delgado, Lidia; Parker, Mango; Fisk, Ian; Paradisi, Francesca (2020). Performance of the extremophilic enzyme BglA in the hydrolysis of two aroma glucosides in a range of model and real wines and juices. Food Chemistry, 323, p. 126825. Elsevier 10.1016/j.foodchem.2020.126825

Romero-Fernández, María; Paradisi, Francesca (2020). Protein immobilization technology for flow biocatalysis. Current opinion in chemical biology, 55, pp. 1-8. Elsevier 10.1016/j.cbpa.2019.11.008

Heckmann, Christian M.; Paradisi, Francesca (2020). Looking back: A short history of the discovery of enzymes and how they became powerful chemical tools. ChemCatChem, 12(24), pp. 6082-6102. WILEY-VCH 10.1002/cctc.202001107

Contente, Martina L.; Roura Padrosa, David; Molinari, Francesco; Paradisi, Francesca (2020). A strategic Ser/Cys exchange in the catalytic triad unlocks an acyltransferase-mediated synthesis of thioesters and tertiary amides. Nature catalysis, 3(12), pp. 1020-1026. Nature Publishing Group 10.1038/s41929-020-00539-0

Roura Padrosa, David; Alaux, Raphael; Smith, Phillip; Dreveny, Ingrid; López-Gallego, Fernando; Paradisi, Francesca (2019). Enhancing PLP-Binding Capacity of Class-III ω-Transaminase by Single Residue Substitution. Frontiers in Bioengineering and Biotechnology, 7(282) Frontiers Media 10.3389/fbioe.2019.00282

Contente, Martina Letizia; Farris, Stefano; Tamborini, Lucia; Molinari, Francesco; Paradisi, Francesca (2019). Flow-based enzymatic synthesis of melatonin and other high value tryptamine derivatives: a five-minute intensified process. Green chemistry, 21(12), pp. 3263-3266. Royal Society of Chemistry 10.1039/C9GC01374A

Planchestainer, Matteo; Hegarty, Eimear; Heckmann, Christian M.; Gourlay, Louise J.; Paradisi, Francesca (2019). Widely applicable background depletion step enables transaminase evolution through solid-phase screening. Chemical Science, 10(23), pp. 5952-5958. The Royal Society of Chemistry 10.1039/C8SC05712E

Contente Martina Letizia, Contente; Paradisi, Francesca (2019). Transaminase-catalyzed continous synthesis of biogenic aldehydes. ChemBioChem, 20(22), pp. 2830-2833. Wiley-VCH 10.1002/cbic.201900356

Planchestainer, Matteo; Ségaud, Nathalie Sylvie Laetitia; Shanmugam, Muralidharan; McMaster, Jonathan; Paradisi, Francesca; Albrecht, Martin (2018). Carbene in Cupredoxin Protein Scaffolds: Replacement of a Histidine Ligand in the Active Site Substantially Alters Copper Redox Properties. Angewandte Chemie (International ed.), 57(33), pp. 10677-10682. Wiley-VCH 10.1002/anie.201807168

Book Section

Paradisi, Francesca; Poppe, László (2021). Continuous-flow biocatalysis with enzymes and cells. In: Darvas, Ferenc; Dormán, György; Hessel, Volker; Ley, Steven V. (eds.) Volume 2 Flow Chemistry – Applications. De Gruyter Textbook (pp. 277-312). De Gruyter 10.1515/9783110693690-010

Romero Fernández, María; Paradisi, Francesca (2020). General overview on immobilisation techniques of enzymes for biocatalysis. In: Benaglia, Maurizio; Puglisi, Alessandra (eds.) Catalyst Immobilization: Methods and Applications (pp. 409-436). Wiley-VCH

Conference or Workshop Item

Paradisi, Francesca (3 March 2020). Progress in Flow Biocatalysts: Multi-enzyme Cascades and Implementation (Unpublished). In: Flow Chemistry Europe 2020.

Paradisi, Francesca (17 February 2020). Versatility of transaminases in flow biocatalysis (Unpublished). In: Amine Biocatalysis 4.0.

Planchestainer, Matteo; Lenzen, Karst Eelco; Paradisi, Francesca; Albrecht, Martin (6 December 2019). Installing a metal active centre in peptide scaffolds thought an unnatural NHC-amino acid (Unpublished). In: CSCB Symposium. Dublin, Ireland. 6.12.2019.

Paradisi, Francesca (6 December 2019). Heterogenous biocatalysis in continuous flow systems as a new approach to synthetic chemistry (Unpublished). In: Recent Advances in Synthesis and Chemical Biology XVIII. Dublin, Ireland.

Paradisi, Francesca (21 November 2019). Biocatalysis and Flow Technology (Unpublished). In: Swiss Industrial Biocatalysis Consortium 2019.

Paradisi, Francesca (21 June 2019). Continuous Flow biocatalysis for highly sustainable and versatile syntheses (Unpublished). In: 71st Irish Universities Chemistry Research Colloquium.

Planchestainer, Matteo; Ségaud, Nathalie Sylvie Laetitia; Paradisi, Francesca; Albrecht, Martin (14 June 2019). Carbene- rather than Nitrogen-metal bonding mode alters metallo-enzymes features (Unpublished). In: NoNoMeCat Symposium. Basel, Switzerland. 14.6.2019.

Planchestainer, Matteo; Lenzen, Karst Eelco; Paradisi, Francesca; Albrecht, Martin (9 June 2019). Installing a metal active centre in peptide scaffolds thought an unnatural NHC-amino acid (Unpublished). In: Swiss Chemical Society Fall Meeting 2019. Zurich, Switzerland. 6.9.2019.

Paradisi, Francesca (19 May 2019). Sustainable Catalysis: enzyme-mediated synthesis of high value chemicals and pharmaceuticals in Flow Reactors (Unpublished). In: 5th IMTB. Cavtat, Croatia.